Analysis Note

ControlHeLa cell lysate

Application

Detect Ribonucleotide Reductase using this mouse monoclonal antibody, Anti-RRM2 Antibody, clone 4D1.2 validated for use in western blotting & ICC.

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RRM2 in HepG2 and A431 cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Ribonucleoside-diphosphate reductase subunit M2 (RRM2) is a cytoplasmic protein, which catalyzes the conversion of ribonucleoside diphosphates to deoxyribonucleoside diphosphates, and plays an important role in DNA damage and repair. RRM2 has been implicated in various types of cancer.

Immunogen

GST-tagged recombinant protein corresponding to human RRM2.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected RRM2 in 10 µg of HeLa cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~45 kDa observed